Taking our selective & differential agar plates, we observed the EMB, Mannitol and MacConkey results for bacteria 'L'. The Mannitol plate was negitive, this means our bacteria did not grow and does not tolerate salt and cannot ferment Mannitol. This means our bacteria is not a halophile (therefore not of the
staphylococcus genus). Our bacteria did grow on the MacConkey agar & was pinkish colored on the EMB agar! This means our bacteria is labeled as gram-negaticve enteric bacteria and partially ferments lactose.
Mannitol
EMB
Hydrogen Peroxide was also used in class & placed onto of the bacteria to test for the enzyme catalyse. As the hydrogen peroxide touched bacteria 'L' bubbles formed meaning that the bacteria successfully broke down the hydrogen peroxide into water and that it is an aerobe (which we knew since ours is facultative) and does contain the enzyme catalyse!!
Identity of 'L' is almost clear!!
Later,
Annie B
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