Today the class took the bacteria out of the fridge to see how much it had grown. We looked at them closer under a microscope as well. My bacteria that was isolated from my throat was white colonies and were raised off the agar plate like little half bumps. There were also a couple yellow colonies by the white colonies. This signifies at least two bacteria types on the plate. Since there is more than one type we needed to create a select one and create a pure culture of it. I chose to create a pure culture of the white bacteria since there was more of it. To create a pure culture we took a inoculating loop and sterilized it with a Bunsen burner. Than we took a swab of one type of bacteria from our petri dishes and created a streak plate. We split the plate into four quadrants and spread the bacteria in a clockwise manner until it was spread out in the petri dish in the four quadrants. The purpose of this was to isolate the bacteria and to make it a pure culture. Once the petri dish was properly spread we labeled it with the date, name and type of sample (environmental). It was than set in the incubator at 25 degrees Celsius.
Master Bacteria Sample (that we took bacteria from for the new streak plate)
The new sample should grow into individual colonies and thus isolate one type of bacteria for a pure culture. This is important because in one speck of bacteria on the plate there are actually millions of bacteria growing there. The spread plate technique also is useful because with each quadrant the bacteria sample becomes more spread out and more isolated.
A Look At The Bacteria Through A Microscope
Streaking The New Petri Dis
Separating The Petri Dish Into Quadrants
Dr. Joseph Sterilizing The Loop In Between Streaking
Hope You Enjoyed,
Annie B
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