Today in lab we discovered how to store bacteria forever! By using Glycerol to slow down the rapid cooling of the bacteria membrnes from the refrigerator (this causes the bacteria membranes to burst). We first used 300 microLiters of Glycerol (100%) using a micropipet. We than placed this into a small storing capsule and replaced the plastic covering on the micropipette to prevent contamination of the bacteria. Than with the new tip we measured 700microLitters of the bacteria in broth and added it to the 100% Glycerol and mixed them together. Once properly labeled we placed the capsule in a -80 degree Celsius refridgertor on the rack that read 'FOR NURSING MAJORS ONLY' so it didn't get confused with Biology majors samples!
We than used Thioglycollate Broth to determine if 'L' was anaerobic or not! This test is positive if the Thioglycollate does not turn red (red is where the chemical reacted with oxygen). Using the aseptic technique, we placed 'L' into the broth and left it in a 25 degree incubator. If the bacteria grows in the yellow portion of the test tube it is anaerobic and if it goes in the red it is aerobic.
Micropipetting The 100% Glycerol
Our bacteria that is going to live FOREVER!
GasPak & Thioglycollate Broth
As a class, we used a GasPak to see if our bacteria was anaerobic. Our sample was placed on a nutrient agar plate (using aseptic technique & inoculating loop) and placed inside the Pak. The Pak eliminates oxygen (as seen on the colorless strip that appears blue when oxygen it present!) Instead CO2 is inside the Pak. If our bacteria 'L' does grow it means that it is anaerobic. Since it has already been thriving in aerobic conditions it really means that it is facultative.
Until Next Lab,
Annie B
Until Next Lab,
Annie B
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