First up is the Gelatin Hydrolysis Test. After placing the same in the refrigerator for 15 minutes we checked the gelatin tube for liquidation. Then we compared it to the control tube we placed in (of just the gelatin). Result: POSITIVE! Our tube did in fact have liquid!! This means that there is gelatinase in bacteria 'L' because it consumed the gelatin!
Test 2 was the fermentationi of carbohydrates! Upon observation it was determined that our bacteria ferments glucose and sucrose but it does not ferment lactose. Test 3 was TSIA and bacteria 'L' had a red slant and yellow butt of slant. There was also a gas bubble that split the butt! (acidic and produces CO2). This means only glucose was fermented. Last but not least was the results for teh MR-VP! Once we pulled the tube out of the incubator we slit it into two more tubes. In one tube we added 5 drops of Methyl Red de and in the other tube we added 5 drops of VP-B and 15 drops of VP-A. Our bacteria was negative fore the Methyl Red Test but Positive for the Voges-Proskauer due to a red band identified near the top of the tube. This means it ferments by Butanediol Fermentation and breaks acids down into non-acids!
Gelatin Hydrolysis Liquidation
Lactose
TSIA (notice the air bubble on the right side of butt)
Sucrose
VP-positive (left) MR-negitive (right)
Glucose & Sucrose
Annie B
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